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Real-time PCR system for detection of Vibrio parahaemolyticus

 

Introduction

PCR technology has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demands for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry than before. GeneReach has developed IQ REAL™ Vibrio parahaemolyticus Quantitative System a real-time PCR-based diagnostic system. Its advanced designs, listed below, provide an accurate, sensitive, and flexible real-time PCR system for Vibrio parahaemolyticus (V. parahaemolyticus) detection in the shrimp culture industry.

Advantages of IQ REAL™ Vibrio parahaemolyticus Quantitative System:

  1. TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/shrimp cell ratio.
  2. Dual color design: This system can quantify both target pathogen (FAM) and shrimp (VIC) genomic nucleic acid in a single reaction. The built-in internal control (VIC) prevents any false negative interpretation of results from failed extraction or inhibition.
 
 
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