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Real-time PCR system for detection of Acute Hepatopancreatic Necrosis Disease (AHPND) / Shrimp Early Mortality Syndrome (EMS)

 

Introduction

PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demand for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry. IQ REAL AHPND/EMS Quantitative System is a real-time based diagnostic system which not only meets the above demands but its advanced designs, listed below, also provides the shrimp culture industry a more accurate, more sensitive and more flexible real-time system.

Advantages of IQ REAL AHPND/EMS Quantitative System

  1. TaqMan® assay strategy: This system can tolerate higher concentration of sample load than SYBR Green. Consequently, it is able to detect the samples with very low bacterial/ shrimp cell ration.
  2. Dual color design: This system can quantify both bacterial (FAM) and shrimp genomic nucleic acid (VIC) by a single reaction. This built-in internal control avoids the false negative results from failed extraction or inhibition.
  3. Absolute and comparative quantitation: This system can quantify the bacterial amount by both absolute quantitative method (Qab, bacterial amount/µl) and comparative method (Qcp, bacterial amount/ 10,000 shrimp cells). The Qcp method can reflect the real infectious level and provide constant result for a sample within wide range of concentration.
  4. No standard required : There is no need to make a calibration curve for Qcp method. A unique ΔΔCt strategy is introduced to interpret the final result. There is no waste for standards as the reagents are only for the use of samples.
  • Technology transfer from the University of Arizona.
  • TaqMan® is the trademark licensed under Applied Biosystems.
 
 
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