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Real-time PCR system for detection of Nervous Necrosis Virus (VNN)

 

Introduction

PCR technology has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demands for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry than before.

GeneReach has developed IQ REAL™ VNN Quantitative System, a real-time PCR-based diagnostic system. Its advanced designs, listed below, provides an accurate, sensitive, and flexible real-time PCR system for VNN detection in the fish farming industry.

Advantages of IQ REAL™ VNN Quantitative System:

  1. TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/fish cell ratio.
  2. Dual color design: This system can quantify both target pathogen (FAM) and fish (VIC) genomic nucleic acid in a single reaction. The built-in internal control (VIC) prevents any false negative interpretation of results due to failed extraction or inhibition.
  3. Absolute and comparative quantitation: This system can quantify the target pathogen by both absolute quantitation method (Qab, target quantity/μl) and comparative quantitation method (Qcp, target quantity/10,000 fish cells). The Qcp method can reflect the actual infection level and provide consistent results for a sample within a wide range of concentration.
  4. No standard required for Qcp method: There is no need to make a calibration curve for Qcp method. Since the ΔΔCt strategy is used to interpret the final results, there is no need to generate a standard curve in each run, saving reagent costs in the long run.
 
 
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