Iridoviruses have caused ecological destruction to wild fish populations and economic impacts on marine fish farming in recent years. They are large icosahedral deoxyriboviruses that can be categorized into at least four genera, which include Iridovirus, Chloriridovirus, Ranavirus, Lymphocystivirus and Megalocytivirus. Pathogenic iridovirues have been isolated from diseased grouper (Epinephelus hybrid), giant seaperch (Lates calcarifer), largemouth bass (Micropterum salmoides), king grouper (Epinephelus lanceolatus), spotted butter fish (Scatophagus argus), yellow-wax pomfrat (Trachinotus blochii), goldlined seabream (Sparus sarba), humpback grouper (Chromileptes altivelis) and Mangrove red snapper (Lutjanus argentimaculatus). Pathogenic iridovirues causes mass mortality in cultured grouper fry.
IQ2000™ Irido Detection and Prevention System is a diagnostic system specially designed to detect Megalocytivirus by GeneReach. This system adopts the design of nested PCR and also inherits the reliability, sensitivity and experiences from a range of IQ2000™ diagnostic kits for shrimp and fish viral infections. IQ2000™ Irido can effectively and reproducibly detect 10 copies of the virus per reaction, while in most cases digit copies of virus can also be detected. IQ2000™ Irido offers you the best tool for the prevention of Iridovirus infection.
Packing size: 200 reactions.
Detection Limit: 10 copies/reaction.
Quantitative capability: 3 different levels of infection can be differentiated.
Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.
Built-in internal control system: eliminate false negative results from failed extraction or reaction.
Quantified positive standard: monitor the sensitivity of detection.
Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.
An example of the results is shown and explained below:
Lane 1: Iridovirus P(+) standard, 2,000 copies/reaction
Lane 2: Iridovirus P(+) standard, 200 copies/reaction
Lane 3: Iridovirus P(+) standard, 20 copies/reaction
Lane 4: Sample of severe Iridovirus infection
Lane 5: Sample of light Iridovirus infection
Lane 6: Iridovirus negative sample
Lane 7: Negative control (Yeast tRNA or ddH2O)
Lane M: Molecular weight markers, 848 bp, 630 bp, 333 bp
This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. gill, larvae, spleen, and kidney.
Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.