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IQ2000 SVCV Detection and Prevention System

 

Introduction

Spring Viremia of Carp (SVC) is a contagious viral disease affecting cyprinid fish. Not only common carp (Cyprinus carpio), infections have also been reported in koi carp (Cyprinus carpio koi), grass carp (Ctenopharyngodon idella), silver carp (Hypophthalamicthys molitrix), bighead carp (Aristicthys nobilis), crucian carp (Carassius carassius), golden fish (Carassius auratus), tench (Tinca tinca), orfe (Leuciscus idus), and sheatfish/European catfish/wels (Silurus glanis).

Until today, the SVC has been reported in Europe including UK, Russia, Belarus, Georgia, Lithuania, Moldova, Ukraine, Middle East, China, USA and Brazil. According to the statistics, in Europe, 10-15% of yearly carps are lost to SVC each year and the mortality has reached 70% in one-year-old carps.

Spring Viremia of Carp is caused by the spring viremia of carp virus (SVCV). It belongs to Rhabdoviridae and is a bullet-shaped RNA virus. Fish can carry SVCV with or without symptoms. The virus enters the fish most often through the gills and replicates in gill epithelium. Because of the longevity of the virus in water, mud, or following desiccation, transmission is thought to be by direct contact or through the water. SVCV isolates can be divided into four genetic groups. And the primer sets in the IQ2000™ SVCV Detection and Prevention System was designed to detect the genogroup IA which originate from Asia.

The IQ2000™ SVCV Detection and Prevention System has adopted the design of nested PCR and also inherited the reliability, sensitivity and experience from a range of IQ2000™ virus diagnostic kits for shrimp and fish virus disease. Its unique semi-quantitative function provides information of virus infection levels which could be used for further understanding on SVCV.

Specifications:

  • Packing size: 200 reactions.
  • Detection Limit: 10 copies/reaction.
  • Quantitative capability: 4 different levels of infection can be differentiated.
  • Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.
  • Built-in internal control system: eliminate false negative results from failed extraction or reaction.
  • Quantified positive standard: monitor the sensitivity of detection.
  • Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

An example of the results is shown and explained below:

Lane 1: SVCV P(+) standard, 2,000 copies/reaction
Lane 2: SVCV P(+) standard, 200 copies/reaction
Lane 3: SVCV P(+) standard, 20 copies/reaction
Lane 4: Negative control (Yeast tRNA)
Lane 5: Sample of severe SVCV infection
Lane 6: Sample of moderate SVCV infection
Lane 7: Sample of light SVCV infection
Lane 8: SVCV negative sample
Lane 9: Molecular weight markers, 848 bp, 630 bp, 333 bp

Applications:

  • This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. gill, spleen, kidney.
  • Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.
 
 
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