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IQ2000 MBV Detection and Prevention System

 

Introduction

Penaeus monodon-type baculovirus (MBV) is one of the earliest found shrimp viruses, but its impact on shrimp farming has yet to be further studied.  MBV was considered to be one of the most critical fatal shrimp diseases for P. monodon in the past, but recent researches have shown no direct association of such disease to the cause of failure in shrimp culture industry in respect of growth retardation, deformation, and mortality rate.

Nevertheless, MBV is still a considerable issue for hatchery management as it may affect the survival rate of PLs. The consequences of severe MBV infection not simply affect the PLs survival rate, but the performance of PLs also turns out to be worse than those lightly infected or negative ones. As the major transmission path of MBV is horizontal, the degree of PLs MBV infection has been taken as a major indicator of hatchery hygiene in some areas.

The comparison of different MBV infected samples from several Asian countries has indicated that the variation of nucleic acid sequence is more than expected. For example, within the published sequence by Craig R. Belcher (J. Virol. Method 74, 1998, p21-29), the homology of the sample from Taiwan is only 91.8%; 92.3% for sample from Thailand, 92.1% for sample from Indonesia, and 94.1% for sample from India. These sequence variations may cause false negative results by following Craig’s protocol.

GeneReach has been researching on MBV continuously. This newly released version of IQ2000 MBV Detection and Prevention System has the broadest coverage of strains in comparison with other known products in the market and has also inherited the built-in internal control design and semi-quantitative data format from a chain of our IQ2000 serial products.  Moreover, we always ensure that our detection system is updated to the most advanced research results in order to provide the best detection tool for all IQ2000TM users.

Specifications:

  • Packing size: 200 reactions.
  • Detection Limit: 10 copies/reaction.
  • Quantitative capability: 3 different levels of infection can be differentiated.
  • Sample throughput: for 40 samples, from sample preparation to final results require 2.5 to 3 hours.
  • Built-in internal control system: eliminate false negative results from failed extraction or reaction.
  • Quantified positive standard: monitor the sensitivity of detection.

An example of the results is shown and explained below:

Lane 1:  MBV P(+) standard, 20,000 copies/reaction
Lane 2:  MBV P(+) standard, 2,000 copies/reaction
Lane 3:  MBV P(+) standard, 200 copies/reaction
Lane 4:  Negative control (Yeast tRNA or ddH2O)
Lane 5:  HP sample of severe MBV infection
Lane 6:  HP sample of light MBV infection
Lane 7:  Fecal sample of MBV infection
Lane 8:  MBV negative sample
Lane M:  Molecular weight markers, 848 bp, 630 bp, 333 bp

Applications:

  • This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. broodstock’s feces, hepatopancreas, and PL.
  • Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

 

 
 
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