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IQ2000 IMNV Detection and Prevention System

 

Introduction

Infectious Myonecrosis Virus (IMNV) had its first appearance in the State of Piaui, Brazil, in September, 2002. And it is not until February, 2004, that Dr. Donald V. Lightner, from the University of Arizona, US, officially confirmed the discovery of the new virus. The late discovery and the lack of information about the pathology caused severe damages to the Brazilian shrimp farming industry. In 2003, loss in production was estimated about $20 million USD in crops of L. vannamei, an unexpected impact for the sector.

The “Zombie Virus”, a nickname given by farmers, is still of an unknown origin. The virus presents RNA genome and belongs to the Totiviridae family. The typical symptoms are necrosis of abdomen and cephalothorax, loss volume of hepatopancreas, loss of transparency and coloration around the tail, an appearance of cooked shrimp, etc. Mortality can occur throughout all cycles of shrimp’s life, and its rate increases when the shrimp is over 6 g, which matches the phase of high consumption of food ration, causing great loss in resources and feed.

In cooperation with the research team leaded by Dr. Donald V. Lightner, GeneReach has created a highly accurate detection system for IMNV, a new product in IQ2000 viral detection series. It has inherited all the same features from IQ2000 series, such as internal control and semi-quantitative design. The first design eliminates false negative possibilities, and the second one measures the degree of infection.


Specifications:

  • Packing size: 200 reactions.
  • Detection Limit: 10 copies/reaction.
  • Quantitative capability: 3 different levels of infection can be differentiated.
  • Sample throughput: for 40 samples, from sample preparation to final results require 4.5 to 5 hours.
  • Built-in internal control system: eliminate false negative results from failed extraction or reaction.
  • Quantified positive standard: monitor the sensitivity of detection.
  • Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

An example of the results is shown and explained below:

Lane 1:  IMNV P(+) standard, 2,000 copies/reaction
Lane 2:  IMNV P(+) standard, 200 copies/reaction
Lane 3:  IMNV P(+) standard, 20 copies/reaction
Lane 4:  Negative control (Yeast tRNA or ddH2O)
Lane 5:  Sample of severe IMNV infection
Lane 6:  Sample of light IMNV infection
Lane 7:  IMNV negative sample
Lane M:  Molecular weight markers, 848 bp, 630 bp, 333 bp

Applications:

  • This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. pleopod, muscle, and PL.
  • Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.

 

 
 
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